pcas α syn gfp Search Results


pcas α syn gfp  (TaKaRa)
95
TaKaRa pcas α syn gfp
Apposition of ER and mitochondria in HeLa cells. A, Examples of colocalization of ER labeled with <t>GFP-Sec61-β</t> (green), and mitochondria labeled with pDsRed2-mito (red), in HeLa cells transiently expressing WT- and <t>A30P-α-syn.</t> B, Quantitation of colocalization (as in A) by ImageJ analysis, normalized to the baseline level of apposition value in EV cells (baseline = 100; average of 4 experiments ±SD). Asterisk denotes significance versus EV.
Pcas α Syn Gfp, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcas α syn gfp/product/TaKaRa
Average 95 stars, based on 1 article reviews
pcas α syn gfp - by Bioz Stars, 2026-03
95/100 stars
  Buy from Supplier

Image Search Results


Apposition of ER and mitochondria in HeLa cells. A, Examples of colocalization of ER labeled with GFP-Sec61-β (green), and mitochondria labeled with pDsRed2-mito (red), in HeLa cells transiently expressing WT- and A30P-α-syn. B, Quantitation of colocalization (as in A) by ImageJ analysis, normalized to the baseline level of apposition value in EV cells (baseline = 100; average of 4 experiments ±SD). Asterisk denotes significance versus EV.

Journal: The Journal of Neuroscience

Article Title: ?-Synuclein Is Localized to Mitochondria-Associated ER Membranes

doi: 10.1523/JNEUROSCI.2507-13.2014

Figure Lengend Snippet: Apposition of ER and mitochondria in HeLa cells. A, Examples of colocalization of ER labeled with GFP-Sec61-β (green), and mitochondria labeled with pDsRed2-mito (red), in HeLa cells transiently expressing WT- and A30P-α-syn. B, Quantitation of colocalization (as in A) by ImageJ analysis, normalized to the baseline level of apposition value in EV cells (baseline = 100; average of 4 experiments ±SD). Asterisk denotes significance versus EV.

Article Snippet: Other expression plasmids used: pCAS-α-syn-GFP (a kind gift from Dr Oren Levy, Columbia University), pDsRed2-Mito (Clontech; no. 632421), and GFP-Sec61-β (Addgene; no. 15108).

Techniques: Labeling, Expressing, Quantitation Assay

Relationship of WT α-syn expression to mitochondrial fragmentation. A, M17 cells stably transfected with an EV plasmid or with the indicated plasmids expressing α-syn species were transiently cotransfected with plasmids expressing pDsRed2-Mito (red) and WT α-syn-GFP (green) and visualized by confocal microscopy. Cells were inspected visually and scored for whether they contained predominantly tubular or predominantly fragmented mitochondria (red). Note that, compared with Figure 7, the fragmented phenotype in the α-syn mutant cells is ameliorated by the expression of WT α-syn-GFP. B, Quantitation of percentage fragmented cells shown in A. *Significant difference versus corresponding untransfected cells (p < 0.05).

Journal: The Journal of Neuroscience

Article Title: ?-Synuclein Is Localized to Mitochondria-Associated ER Membranes

doi: 10.1523/JNEUROSCI.2507-13.2014

Figure Lengend Snippet: Relationship of WT α-syn expression to mitochondrial fragmentation. A, M17 cells stably transfected with an EV plasmid or with the indicated plasmids expressing α-syn species were transiently cotransfected with plasmids expressing pDsRed2-Mito (red) and WT α-syn-GFP (green) and visualized by confocal microscopy. Cells were inspected visually and scored for whether they contained predominantly tubular or predominantly fragmented mitochondria (red). Note that, compared with Figure 7, the fragmented phenotype in the α-syn mutant cells is ameliorated by the expression of WT α-syn-GFP. B, Quantitation of percentage fragmented cells shown in A. *Significant difference versus corresponding untransfected cells (p < 0.05).

Article Snippet: Other expression plasmids used: pCAS-α-syn-GFP (a kind gift from Dr Oren Levy, Columbia University), pDsRed2-Mito (Clontech; no. 632421), and GFP-Sec61-β (Addgene; no. 15108).

Techniques: Expressing, Stable Transfection, Transfection, Plasmid Preparation, Confocal Microscopy, Mutagenesis, Quantitation Assay